Rapid Screening Of A Cell-Based Assay For GLP-1 Receptor Using A Natural Product Library
By Brad Larson and Peter Banks, BioTek Instruments, Inc. and Nicolas Pierre, Suzanne Graham, Jean-Luc Tardieu, and François Degorce, Cisbio US, Inc.
Here we demonstrate the detection of GLP-1R binding through the incorporation of a homogeneous cell-based binding assay using HTRF Tag-lite® technology. The assay resembles classical radiometric ligand receptor binding, except that it uses a red-emitting labeled ligand and transfected cells. When the ligand binds to the receptor, an increase in HTRF signal may be measured. Competitive binding of non-labeled ligands or inhibitors causes a subsequent decrease.
The assay was automated using a non-contact liquid dispenser commonly used in high throughput settings.
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