Application Note: Quantitation Of Human TNF-α With A QuantiGlo Assay From R&D Systems Using BioTek's Synergy 2

By Paul Held Ph. D., Senior Scientist, Applications Dept., BioTek Instruments, Inc.

The peptide TNF-α is a cytokine that plays a key role in inflammation and apoptosis. As such, quantitation of this cytokine from tissue culture lysates and from human serum and/or plasma samples under experimental and environmental conditions can provide important information regarding the regulation of these phenomenons. Here we describe the quantitation of TNF-α using a Synergy™ 2 Multi-Mode Microplate Reader form BioTek Instruments to measure the luminescence signal of a QuantiGlo kit from R&D Systems.