QArray Family: RNAi And Microarrays
Source: Genetix Limited
Click Here To Download:
•Application Note: QArray Family: RNAi And Microarrays
Over 10 years ago a post-transcriptional gene silencing mechanism was discovered in petunias. Since this observation a similar mechanism has been discovered in many other organisms, including insects, nematodes and mammals, and has become known under the general name of RNA Interference (RNAi). The mechanism operates via the interaction of double-stranded short interfering RNA structure or siRNA (typically 20-25 nucleotides) with the complementary target mRNA and specific proteins to form an RNA-induced silencing complex (RISC). The siRNA molecules themselves are generated from longer transcripts by the enzyme dicer. This naturally occurring mechanism has been developed as a powerful molecular biology tool to achieve targeted gene silencing. In mammalian cells the dsRNA can be introduced in several forms, most usually as synthetic RNA oligonucleotides or via ssRNA that can form a hairpin. The stem of the hairpin (shRNA) forms the dsRNA necessary for RNAi to occur. An advantage of the second mechanism is that the ssRNA can be produced within the cell as a transcript from an introduced plasmid or viral vector.
•Application Note: QArray Family: RNAi And Microarrays
SOURCE: Genetix Limited
•Application Note: QArray Family: RNAi And Microarrays
Over 10 years ago a post-transcriptional gene silencing mechanism was discovered in petunias. Since this observation a similar mechanism has been discovered in many other organisms, including insects, nematodes and mammals, and has become known under the general name of RNA Interference (RNAi). The mechanism operates via the interaction of double-stranded short interfering RNA structure or siRNA (typically 20-25 nucleotides) with the complementary target mRNA and specific proteins to form an RNA-induced silencing complex (RISC). The siRNA molecules themselves are generated from longer transcripts by the enzyme dicer. This naturally occurring mechanism has been developed as a powerful molecular biology tool to achieve targeted gene silencing. In mammalian cells the dsRNA can be introduced in several forms, most usually as synthetic RNA oligonucleotides or via ssRNA that can form a hairpin. The stem of the hairpin (shRNA) forms the dsRNA necessary for RNAi to occur. An advantage of the second mechanism is that the ssRNA can be produced within the cell as a transcript from an introduced plasmid or viral vector.
Click Here To Download:
•Application Note: QArray Family: RNAi And Microarrays
SOURCE: Genetix Limited
This website uses cookies to ensure you get the best experience on our website. Learn more