Pyrochrome® Kinetic Chromogenic Endotoxin Testing

Pyrochrome is a versatile quantitative reagent that is used to perform kinetic or endpoint assays. It is a sensitive and flexible reagent that can be used for testing in compliance with the USP, EP and JP bacterial endotoxins test chapter. Pyrochrome is offered with either Pyrochrome Buffer or for endotoxin-specific testing, with Glucashield® Buffer.

Pyrochrome can be used with the new Pyros Kinetix® Flex tube reader at a 1:1 and economical 4:1 sample to LAL ratio. Pyrochrome can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference. The standard Pyrochrome test is read at 405–410 nm.

It is also offered in a diazo kit for endpoint tests. (The diazo reagents shift the absorption spectrum making it especially useful for testing samples with color interference.) The test is read at 540–550 nm.

Sensitivity
The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Pyrochrome is 0.001 EU/mL when run in Pyros Kinetix Flex tube reader or incubating microplate reader with Glucashield Buffer.

Catalog#:

#C1500 - - - - - - - - - - - - - - - - - - - - - - - 60 Test
#CD060 - - - - - - - - - - - - - - - - - - - - - - - 60 Test
(with Diazo-reagents)
#CG1500 - - - - - - - - - - - - - - - - - - - - - - 60 Test
(with Glucashield Buffer)