Protein A Resin: Minimize The Impact Of Elution Buffer Selection

Protein A chromatography is widely used as the affinity capture step of both mAbs and Fc-fusion proteins because of its high degree of selectivity. Variations in elution behavior of the protein A capture step requires more process development work and could have an impact on the polishing step in the downstream process. Thus minimizing the variation in elution pH between different molecules for example, makes it easier to platform the capture process.