Monoclonal (Mouse) Anti-Cain

Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin (Cat. # MA3-917 and MA3-918), FKBP12 (Cat. # PA1-026), the ryanodine receptor (Cat. # MA3-916 and MA3-925), inositol 1,4,5-trisphosphate (Cat. # PA1-901, PA1-904, PA1-905, PA3-901), and a recently identified protein, calcineurin inhibitor (cain).

Cain has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown cain to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of cain to amphiphysin 1, however, does not affect amphiphysin 1's interaction with other endocytic proteins. Cain, like calcineurin, has high neuronal expression and a wide tissue distribution.

PA3-026 has been successfully used in immunoprecipitation and Western blot procedures to detect rat cain. Immunoprecipitation of cain in rat brain tissues shows an interaction between cain and amphiphysin 1, adaptin (Cat. # MA1-064 and MA3-061), and dynamin 1 (Cat. # PA1-660). By Western blot this antibody detects an ~240 kDa band in rat liver, brain, spleen, lung, kidney and testis representing cain. It does not detect cain in rat heart or skeletal muscle when message is also not detected.

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