Microbial Fermentation In Single-Use Xcellerex™ XDR-50 MO Fermentor SystemSource: GE Healthcare Life Sciences
This application note describes the performance of a single-use Xcellerex XDR-50 bioreactor system when used in cultivations of E. coli and of modified Pseudomonas fluorecsens (P. fluorescens) producing a monoclonal antibody (MAb). Both the achieved microbe densities and product yield were shown to be consistent with the performance of conventional stainless steel systems.
The adoption of single-use technology by biomanufacturers using microbial systems has been inhibited by the lack of single-use bioreactor systems capable of accommodating the unique requirements of microbial cultures. Bioreactors designed for production in mammalian culture fall short of meeting these unique requirements, for example in terms of oxygen transfer capacity and temperature control. The Xcellerex XDR-50 MO fermentor system is purpose-designed and built to overcome the mammalian single-use system limitations in fulfilling these needs.