How CHT Ceramic Hydroxyapatite Works Application Note

CHT ceramic hydroxyapatite is a versatile chromatography support used for separation of biological molecules as diverse as polyclonal and monoclonal antibodies, antibody fragments, enzymes, nucleic acids, and membrane proteins. The interactions that occur between CHT and the molecules it binds are complex; this article aims to clarify the most significant features of these interactions.

CHT, Ca₁₀(PO₄)₆(OH)₂, is a mixed-mode support with functional groups consisting of pairs of positively charged crystal calcium ions (C-sites) and clusters of six negatively charged oxygen atoms associated with triplets of crystal phosphates (P-sites). The C-sites, P-sites, and hydroxyl groups are distributed in a fixed pattern on the CHT crystal structure, as presented in classic studies by Kawasaki (1987a, 1978b) and Kawasaki et al. (1985).