GFC-Arrays™: Quick And Reliable Screening For Regulator Genes Via High-throughput Functional Perturbation

Discovery of the key genes regulating a cellular process, a disease progression or a biological pathway has always been a major interest for most biomedical researchers. For decades, scientists have relied heavily on two main approaches. Some search for targets by transfecting entire expression libraries into appropriate cells to screen for genes that produce a desired readout via functional complementation. Due to high library complexity and low target gene transcript abundance, this process is laborious and insensitive. The second approach is to functionally test individual candidate genes one by one. This is an extremely slow process requiring years of data collection, and the chance for ultimate success is low. Identifying and validating novel targets has become an increasingly difficult task, and the research community awaits innovative technologies to advance the discovery process.

OriGene's GFC-Array (Genome Wide Full-length cDNA Arrays) is a revolutionary tool that allows scientists to break through the technical barriers associated with traditional target identification. It enables quick, sensitive and reliable identification of key genes in a cellular process of interest. As the world's largest source of full-length human cDNA clones, OriGene is in the unique position to convert its TrueClone Collection, a collection containing over 24,000 expression-ready cDNAs, into functional screening cDNA arrays. In a GFC array, hundreds or thousands of individual full-length cDNAs are arrayed into a multi-well format. Each well contains 60 ng of a single expression-ready cDNA, a concentration optimized for transfection into a variety of cells used in functional activity assays. A researcher can simply add cells to the GFC plate and apply his/her reporter assays. Once a well shows a distinct readout, the gene in that well can be immediately identified as the underlying cause for the functional perturbation.