All-In-One: Wash Cultured Cells, Vacuum Filtration & Magnetic Bead Separation

This technical note describes how the Tecan HydroFlex platform was successfully evaluated for gentle washing of strongly adherent cell-line A431 as well as for the weakly adherent P815 cells in a 96-well format.

Tecan’s new HydroFlex platform offers advanced features for a range of applications including vacuum filtration, magnetic bead separation and washing of microplates.

For gentle cell washing, the HydroFlex platform offers individual control of critical wash parameters such as speed settings for aspiration and dispense, as well as wash head positioning, allowing fine-tuning of the wash protocol to the specific cell-type used.

A very gentle drop-wise dispense mode combined with a move function optimizes the wash head position relative to the rising liquid level in the wells, thus minimizing cell detachment and ensuring good wash results, even with weakly adherent cell types.