Product/Service

Gene Profile Assays

Source: Xenometrix, Inc.
Gene profiling is the process whereby the status of gene expression in a given cell line is assessed at increasing concentrations of exposure to a test substance (such as a pharmaceutical).
Gene profiling is the process whereby the status of gene expression in a given cell line is assessed at increasing concentrations of exposure to a test substance (such as a pharmaceutical). Xenometrix Gene Profile Assays (GPA) assess gene expression through the use of cell-based assays and specific reporter constructs. These constructs report the activity of certain genes in a quantifiable process, determined at the conclusion of the exposure period. Through the assessment of the activity of key genes, information on the biological activity of the test compound can be gathered, and by including genes relevant to safety or efficacy concerns in the assay, the assay itself can be focused on these critical areas.

Gene Profile Assays in Human Liver Cells

Using the human liver cell line HepG2, and a battery of 13 reporter constructs, this assay generates Gene Profiles for fundamental pathways of biological activity and toxicity. Cellular events such as DNA damage, protein perturbation, cell cycle perturbation, oxidative stress, metal ion contamination, aromatic hydrocarbon metabolism, signal transduction and retinoid activity can all be assessed. Additional constructs are available, and the assay continues to be upgraded as new constructs relevant to safety and efficacy come on line.

Gene Profile Assays in Human Colon Cells

This assay uses a human colon cell line as host to 7 reporter constructs focused on the assessment of DNA damage, and DNA damaging agents. Additional constructs are available, and the assay continues to be upgraded as new constructs relevant to safety and efficacy come on line.

Gene Profile Assays in Bacterial Cells

By using the bacterium E.coli to host a battery of 13 reporter constructs, this Gene Profile Assay enables the user to assess fundamental processes of toxicity that are relevant to both bacteria and mammalian cells. The construct battery covers the assessment of biological activities and toxicity associated with: DNA damage, oxidative stress, protein perturbation, and metal contamination.

Xenometrix, Inc., 2425 North 55th Street, Boulder, CO 80301-5700. Tel: 303-447-1773; Fax: 303-447-1758.