Article | June 13, 2013

Efficient and Cost-Effective Removal Of Residual Endotoxin

By Ben Galarza, Jason Shipp, Buzz Lobbezoo, Jennie Walden and Steve Burton, ProMetic BioSciences Ltd

Endotoxin or lipopolysaccharides (LPS) are highly toxic components of the cell wall of Gram-negative bacteria and are often present in significant amounts in bacterial cell culture expression systems such as E. coli.

A number of methods have been adopted for the removal of endotoxin based on adsorption, in particular ion-exchange chromatography. Although downstream processing can significantly reduce endotoxin levels in the product, efficient and cost-effective removal of residual endotoxin from biopharmaceutical preparations remains a challenge.

This technical poster addresses the issues of removal of endotoxin from biological preparations. Specific reference will be made to a new synthetic ligand affinity adsorbent, EtoxiClear™, which exhibits high affinity for endotoxin, low protein binding and can be depyrogenated using sodium hydroxide.

For full access to this content, please Register or Sign In.

Access Content Efficient and Cost-Effective Removal Of Residual Endotoxin
Newsletter Signup
Newsletter Signup
By clicking Sign Me Up, you agree to our Terms and that you have read our Privacy Policy.