Culturing Of Sf 9 Insect Cells With The Baculovirus Expression Vector System, And SEAP Production

The BEVS (baculovirus expression vector system) is a popular system for producing recombinant proteins in insect cells. This study analyzes the cultured Sf9 insect cell line infected with a baculovirus expressing secreted alkaline phosphatase (SEAP), and compares the growth behavior and productivity in different orbitally shaken culture vessels.

Preliminary experiments were performed to determine and then apply suitable infection and harvesting conditions for the virus, as well as corresponding culturing parameters for different culturing systems. For comparison of the cell cultures, the results of preliminary experiments for purposes of process engineering were used and optimal culturing conditions were defined for three different culturing systems. Explore the results of this study and the performance of the Multitron Incubator Shaker.