Confocal Module

• Analyze 3D image stacks obtained from confocal microscopes

• Perform detailed morphometric analysis on confocal specimens

• Perform 3D neuron reconstruction and analysis on confocal specimens

• Perform stereological analysis on confocal specimens

• Work with structures contained in sets of multiple image stacks

The confocal module is an extension to the Neurolucida and Stereo Investigator programs that provides you with the additional capability of acquiring data from confocal image stacks or from stacks of de-convolved images. The confocal module can read confocal image files in the Biorad PIC format, Olympus Flowview format, as well as image stacks comprised of a series of TIFF, JPEG, or BMP files. All of the capabilities of the standard Neurolucida and Stereo Investigator programs are still available when using the confocal module.

The confocal module allows you to focus through a confocal image stack in real-time. You may specify the exact focal distance between image planes. The module automatically keeps track of the depth (Z-axis) values while you trace. This allows you to perform such tasks as 3D neuron reconstruction and stereology with the same ease as with a standard brightfield microscope. There is no limit to the number of images in a stack. You may even work with structures that are contained in multiple image stacks, allowing you to break the "single stack boundary".

For many investigators, the confocal module has permitted the first extensive morphometric analysis of their confocal images. If you currently view specimens with a confocal microscope, even difficult to analyze structures such as neurons, nerve fibers, or capillaries—the confocal module will add the quantitative dimension to your research

MBF Bioscience, Inc. (MicroBrightField, Inc.), 74 Hegeman Ave., Colchester, VT 05446. Tel: 802-655-9360; Fax: 802-655-5245.