Comparing Single-Use Bioreactors To Reusable Bioreactors For Production Of Recombinant Proteins

By Taylor Hatton, Shaun Barnett, and Kamal Rashid

The objective of this study was to compare Eppendorf BioBLU single-use packed-bed bioreactor vessel and the traditional glass vessel counterpart used in New Brunswick™ CelliGen® 310. Alkaline phosphatase (ALKP)- secreting Chinese Hamster ovary (CHO) cells were used to measure ALKP production in each bioreactor. Overall, the results from these comparisons suggest that there is no significant difference between the reusable and singleuse FibraCel basket systems for bench-scale production of recombinant proteins. Productivity of cells and collection of secreted proteins will not be hindered by the implementation of single-use bioreactor systems.