Product/Service

Column Selection

Source: IDEX
Column selection can help to develop reproducible, rugged methods (frequently validated) in the shortest possible time. Automated column selection can dramatically cut time, reduce cost, and increase productivity while improving analytical results

Column selection can help to develop reproducible, rugged methods (frequently validated) in the shortest possible time. Automated column selection can dramatically cut time, reduce cost, and increase productivity while improving analytical results. With a variety of columns from which to choose, any laboratory can optimize selectivity (different phases), resolution (different particle sizes), time (different lengths), and load/sensitivity (different diameters, injection volumes, and concentration strategies) for all users.

An automated column selector allows multiple users to select methods using the correct column for their assay and convenient washout and column storage. Columns are conveniently stored in the storage solvent of choice requiring no manual changes. Different types of samples can be run at night and over weekends using different methods resulting in better utilization of existing equipment and increased productivity.

In a validated environment, column selectors can be used for column to column reproducibility, method ruggedness, cross method conformational purity analysis on columns with different selectivity, sample long-term stability, solute specificity, column equilibration, and column quality control.

Rheodyne Column Selectors incorporate our patented TeleFLO™ architecture. This unique design eliminates unswept volume assuring no carryover during automated column switching.

Several applications in which column selection is beneficial:

  • SPE Sample Preparation: Two automated 10-column selectors can automate a sample preparation process using cartridge columns.
  • On-line Desalting and Buffer Exchange: selected fractions from one column can be diverted onto a trapping column for subsequent desalting.
  • Chiral Chromatography: Chirality screening requires the use of various methods for optimizing selectivity. Automated column selection increases productivity by allowing multiple columns to be ready in stand-by while one column is on-line.
  • Multidimensional Chromatography: An initial separation mode column, such as Size Exclusion Chromatography (SEC), can be mated to a column selector to divert selected peaks onto a second dimension separation using a different separation mechanism. Examples of secondary mechanisms include ion exchange and hydrophobicity.