Challenges And Solutions Of Scalable Cultivation For Anchorage Dependent Cells In Single-Use BioreactorSource: PBS Biotech, Inc.
By Brian Lee, Yas Hashimura, Daniel Giroux, Marcos Sousa, Joao Clemente, Manuel Carrondo, Siddharth Gupta, Michael McCaman, and Yong-Jae Kim
Microcarrier (MC)-based suspension culture processes have been used to culture anchorage-dependent cells in bioreactors. However, scale-up of the process for high cell growth and productivity poses many challenges such as:
- Achieving reliable cell attachment to MCs during seeding
- Maintaining consistent physical and chemical environment
- Achieving homogeneous MC suspension in a vessel with low power input and low shear stress to the cells
The Vertical-Wheel single-use bioreactor system utilizes a vertically-oriented impeller to impart low shear stress while achieving efficient liquid mixing and homogeneous MC suspension. These studies highlight the benefits of the PBS Vertical-Wheel mixing technology for anchorage dependent cell cultivation for both vaccine and cell therapy applications as a scalable solution for MC-based processes.