By Brian Lee, Yas Hashimura, Daniel Giroux, Marcos Sousa, Joao Clemente, Manuel Carrondo, Siddharth Gupta, Michael McCaman, and Yong-Jae Kim
Microcarrier (MC)-based suspension culture processes have been used to culture anchorage-dependent cells in bioreactors. However, scale-up of the process for high cell growth and productivity poses many challenges such as:
The Vertical-Wheel single-use bioreactor system utilizes a vertically-oriented impeller to impart low shear stress while achieving efficient liquid mixing and homogeneous MC suspension. These studies highlight the benefits of the PBS Vertical-Wheel mixing technology for anchorage dependent cell cultivation for both vaccine and cell therapy applications as a scalable solution for MC-based processes.