BIOXYTECH 8-Isoprostane Assay-Immunoassay for 8-epi-Prostaglandin F2a

Isoprostanes are prostaglandin-like compounds that are produced upon peroxidation of lipoproteins. 8-epi-prostaglandin F2a (8-EPI) has been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs. Isoprostanes may play a role in atherogenesis. Measurement of isoprostane concentrations may be helpful in assessment of oxidative stress, hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis.

Principle:

This assay is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 8-EPI in biological samples. Briefly, 8-EPI in the samples or standards competes for binding (to the antibody coated on the plate) with 8-EPI conjugated to horseradish peroxidase (HRP). The peroxidase activity results in color development in the substrate when added. The intensity of the color is proportional to the amount of 8-EPI-HRP bound and inversely proportional to the amount of 8-EPI in the samples or standards.

Contents of Reagent Box

1) 96 well microtiter plate precoated with anti-8-EPI 8-EPI STANDARD
2) 5 µL WASH BUFFER
3) 5x concentrated
4) 40 mL DILUTION BUFFER
5) 5x concentrated
6) 100 mL SUBSTRATE
7) 25 mL 8-EPI-HRP-CONJUGATE
8) 6 µL 2 Disposable reagent troughs for a multichannel pipettor
9) Instruction booklet Microtiter plate template

For Research Only

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