Benefits Of A Revised Approach To Anion Exchange Flow-Through Polish Chromatography

By Shelly Cote Parra, Christine Gebski, BioPharm International Supplements

Anion exchange (AEX) products are commonly used as a polish step in product flow-through (FT) mode to bind impurities. Comparing resins with membranes and membranes to membranes is challenging due to the complicated and unique scale-down model formats of the products offered by different vendors. A novel approach to AEX FT using a short, 5 cm length, packed bed format with a faster operating flow rate is explained. The performance of commonly used AEX resins and membrane adsorbers, detailing dynamic binding capacity performance, efficiency, and a new disposable option for packed bed chromatography are compared. The data presented will show that a high performance AEX resin competes well with the performance of membranes. It provides similar processing times and the added benefits of reusability, ease of packing at different scales in various column formats, and the ability to implement initial process design from early phase manufacturing to commercial manufacturing, reducing overall costs and time to market.