Product/Service

BalanCD™ CHO Feed 3

Source: Irvine Scientific

Rapid Chemically-Defined Feed Media Development to Improve Simulated Cell Culture Processes

CHO Feed 3 is a chemically-defined protein-free feed medium of non-animal origin. Using Ir vine Scientific’s Rational Culture Media Design™ approach, this formulation has been developed to achieve high growth kinetics and product yield in fed-batch processes.

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CHO Feed 3 is a chemically-defined protein-free feed medium of non-animal origin. Using Irvine Scientific’s Rational Culture Media Design™ approach, this formulation has been developed to achieve high growth kinetics and product yield in fed-batch processes. The feed supplement contains balanced concentrations of glucose, amino acids, vitamins and trace elements to provide nutrients to leaner batch media that are needed to boost the productivity of the cell culture system in fed-batch. The BalanCD™ CHO Feed 3 has been successfully tested on a variety of Chinese Hamster Ovary (CHO) cell lines, including CHO-S, CHO-M, and CHO-dHFR.

BalanCD™ CHO Feed 3 is a complete feed medium that is pH and osmolality pre-adjusted. The dry powdered feed formulation dissolves fast, resulting in a highly concentrated liquid feed that remains stable in solution for extended time. The feed medium is available in a ready-to-use (1X) liquid format with at least 6 month stability when stored under recommended conditions.

PRODUCT HANDLING & STORAGE
Product handle using aseptic techniques is required to avoid contamination. Do not use if cloudy or if solution has precipitated. Powder product should be stored at 2-8°C and in absence of light for up to 6 months. Liquid product should be stored at 2-8°C and in absence of light for up to 1 year. Extended stability studies for both powder and liquid are ongoing for this formulation. Please contact your Ir vine Scientific for additional information if required.

RELATED PRODUCTS
The following products are available from Irvine Scientific and developed for use in CHO fed-batch production systems. For additional information, please visit our website at www.irvinesci.com or call Customer Service at (800) 427-5706.

INTENDED USE
These products are for research use only and not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.

PERFORMANCE STUDY DESIGN
Three diverse model cell lines (CHO-S, CHO-M, and CHO-DHFR) were grown in commercially available competitor growth media. The following culture parameters were applicable to all experimental conditions:

  • 10% (v/v) bolus feeds on day 1,3,5
  • Two (2) replicates per condition
  • Shake flask cultures at 37°C, 5% CO2, 120rpm

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