Product/Service

BalanCD® CHO Feed 2: Chemically-Defined Feed Medium

Source: Irvine Scientific

Rapid Chemically-Defined Feed Media Development to Improve Simulated Cell Culture Processes

BalanCD® CHO Feed 2 is a chemically-defined protein-free feed medium designed to be added as a fed-batch supplement to promote cell growth and increase process yields of antibodies and recombinant proteins.

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BalanCD® CHO Feed 2 is a chemically-defined protein-free feed medium designed to be added as a fed-batch supplement to promote cell growth and increase process yields of antibodies and recombinant proteins. BalanCD CHO Feed 2 improves productivity by providing essential cell culture nutrients such as glucose, amino acids and vitamins.This formulation was developed using Irvine Scientific’s Rational Culture Media Design™ strategy to overcome metabolic overflow which is frequently observed when supplementing richer growth media in fed-btach systems.The BalanCD CHO Feed 2 has been tested with three model cell lines (CHO-S, CHO-M, and CHO-DHFR).

BalanCD CHO Feed 2 is comprised solely of animal component-free, chemically defined components, contains no antibiotics or antimycotics, and has proven stability in both powder and liquid formats.

Product Handling & Storage
Product handling using aseptic techniques is required to avoid contamination. Do not use if cloudy or if solution has precipitated. Powder product should be stored at 2-8°C and in absence of light for up to 6 months. Liquid product should be stored at 2-8°C and in absence of light for up to 1 year. Extended stability studies for both powder and liquid are ongoing for this formulation.

Related Products
The following products are available from Irvine Scientific and developed for use in CHO fed-batch production systems. For additional information, please visit our website at www.irvinesci.com or call Customer Service at (800) 427-5706.

Intended Use
These products are for research use only and not approved for human or veterinar y use, for application to humans or animals, or for use in clinical or in vitro procedures.

Performance
Study Design

The study consisted of three model cell lines (CHO-S, CHO-M, and CHO-DHFR) grown in fed-batch mode using a commercially available competitor growth media paired with a feed media (BalanCD CHO Feed 2 or one of several available commercial feeds) for comparative purposes.The following culture parameters and experimental conditions applied to all:

  • 10% (v/v) bolus feeds on days 1, 3, and 5
  • Two (2) replicates per condition
  • Shake flask cultures at 37°C, 5% CO2, 120rpm Antibody production was measured from samples taken on Day 13 and when viability <75%.

 

 

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