Product/Service

BalanCD® CHO Feed 1: Chemically-Defined Feed Medium

Source: Irvine Scientific

BalanCD® CHO Feed 1 is a chemically-defined protein-free feed medium designed to provide cell culture nutrients for fed-batch applications in conjunction with BalanCD® CHO Growth A.

BalanCD® CHO Feed 1 is a chemically-defined protein-free feed medium designed to provide cell culture nutrients for fed-batch applications in conjunction with BalanCD CHO Growth A. The feed supplement has been developed to promote cell growth and MAb production in a variety of Chinese Hamster Ovary (CHO) cells. This formulation was developed using Ir vine Scientific’s Rational Culture Media Design™ approach to carefully balance key nutrients that provide superior yields in CHO fed-batch applications.

BalanCD CHO Feed 1 is comprised solely of animal component-free, chemically defined components, contains no antibiotics or antimycotics, and has proven stability in both powder and liquid formats.

Product Handling & Storage
Product handle using aseptic techniques is required to avoid contamination. Do not use if cloudy or if solution has precipitated. Powder product should be stored dry at 2-8°C and in absence of light for up to 2 years. Liquid product should be stored at 2-8°C and in absence of light for up to 6 months. Extended stability studies for both powder and liquid are ongoing for this formulation.

Related Products
The following products are available from Ir vine Scientific and developed for use in CHO fed-batch production systems. For additional information, please visit our website at www.ir vinesci.com or call Customer Service at (800) 427-5706.

Intended Use
These products are for RESEARCH USE only and not approved for human or veterinar y use, for application to humans or animals, or for use in clinical or in vitro procedures.

Performance
Study Design
The study consisted of three model cell lines (CHO-S, CHO-M, and CHO-dhfr) grown in fed-batch mode using Irvine Scientific’s BalanCD CHO Growth A and Feed 1 evaluated in a side by side comparative with several commercially available growth and feed media.The following culture parameters and experimental conditions applied to all:

  • 10% (v/v) bolus feeds on days 1, 3, and 5
  • Two (2) replicates per condition
  • Shake flask cultures at 37°C, 5% CO2, 120rpm