Affinity Purified anti-Frequenin

Code: 600-901-216

Lot # 7640

Size: 1.0 mg

Physical State: Liquid (sterile filtered)

Antibody Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01% (w/v) Sodium Azide

Application(s): Suitable for ELISA, immunoblotting, immunohistochemistry, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Recommended Dilution(s): This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Goat) code #603-1302. Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Optimal titers for other applications should be determined by the researcher.

Storage Conditions: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Purity: This product was prepared from chicken egg yolk by selective precipitation followed by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.

Specificty: This affinity purified polyclonal antibody reacts with frequenin from most mammalian sources. Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22kDa when assayed by SDS-PAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. Reactivity with avian and invertebrate frequenin is likely. In Drosophila frequenin is related to recoverin and visinin and functions like Ca²⁺-sensitive guanylyl cyclase activator.

Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)

Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. *Frequenin is the term used to describe the invertebrate homolog of NCS-1 (neuronal calcium sensor-1). The term NCS-1 has been reserved for the protein observed in humans and other mammals, whereas frequenin has been used to describe the homolog in Drosophila and Xenopus.

Rockland Immunochemicals Inc., P.O. Box 316, Gilbertsville, PA 19525. Tel: 610-369-1008; Fax: 610-367-7825.